B. 12. Mutagenicity – in Vivo Mammalian Erythrocyte Micronucleus Test
ثبت نشده
چکیده
When a bone marrow erythroblast develops into a polychromatic erythrocyte, the main nucleus is extruded; any micronucleus that has been formed may remain behind in the otherwise anucleated cytoplasm. Visualisation of micronuclei is facilitated in these cells because they lack a main nucleus. An increase in the frequency of micronucleated polychromatic erythrocytes in treated animals is an indication of induced chromosome damage.
منابع مشابه
Genotoxicity assessment of Elaeagnus angustifolia L. fruit extract (senjed) nanocapsule by in vitro and in vivo methods
Background: Although initial studies on Elaeagnus Angustifolia L. Fruit Extract (Senjed) Nanocapsule showed its efficacy in osteoporosis in rat model as well as its significant role in elevating the serum calcium levels, there is no study on its possible genotixic potentials which is necessary for developing all nanopharmaceuticals. Objective: This study aimed to evaluate the genotoxicity of Se...
متن کاملMutagenicity and genotoxicity studies of arruva, an R,R-monatin salt isomer.
Arruva, the R,R-isomer of monatin (sodium/potassium 2R,4R-2-amino-4-carboxy-4-hydroxy-5-(3-indolyl) pentanoate), an intense sweetener originally identified in root bark of the South African shrub Schlerochitin ilicifolius, was examined for its mutagenic and genotoxic potential via bacterial reverse mutation, mouse lymphoma and in vivo mouse micronucleus assays, all accomplished in the presence ...
متن کاملGenotoxicity evaluation of Oryeong-san water extract using in vitro and in vivo tests
BACKGROUND Oryeong-san, a mixture of five herbal plants, is a well-known therapy for renal-associated diseases such as those manifesting edema, dysuria, and oliguria. METHODS In the present study, we investigatee the potential genotoxic effects of a water extract of Oryeong-san (ORSE) in three mutagenicity assays (an in vitro bacterial reverse mutation assay (Ames test) with Salmonella typhim...
متن کاملAbsence of genotoxic effects of the chalcone (E)-1-(2-hydroxyphenyl)-3-(4-methylphenyl)-prop-2-en-1-one) and its potential chemoprevention against DNA damage using in vitro and in vivo assays
The chalcone (E)-1-(2-hydroxyphenyl)-3-(4-methylphenyl)-prop-2-en-1-one), or 2HMC, displays antileishmanial, antimalarial, and antioxidant activities. The aim of this study was to investigate the cytotoxic, genotoxic, mutagenic, and protective effects of 2HMC using the Ames mutagenicity test, the mouse bone marrow micronucleus test, and the comet assay in mice. In the assessment using the Ames ...
متن کاملToxicological evaluation of fucoidan from Undaria pinnatifidain vitro and in vivo.
The potential toxicity of fucoidan from Undaria pinnatifida was investigated in vitro and in vivo. By the Ames test, fucoidan showed no mutagenicity up to 500 microL/plate, and inhibited the mutagenicity induced by 4-nitro-quinoline-1-oxide, by up to 71%, compared with controls. In the bone marrow micronucleus test, fucoidan, at all levels tested, did not change the micronucleated polychromatic...
متن کامل